This research examines the integration of Indian Knowledge Systems (IKS)—encompassing Vastu Shastra, Ayurveda, Vedic philosophy, and traditional community models—into the sustainable design of electric two-wheelers (E2Ws), aiming to pioneer culturally attuned, eco-harmonious mobility solutions for India's diverse urban-rural landscapes. As electric two-wheelers surge toward 30% market penetration by 2030 under initiatives like PM E-DRIVE, conventional designs overlook holistic human-nature synergy; this study bridges that gap by fusing ancient wisdom with modern engineering for enhanced sustainability, user well-being, and adoption. Core contributions include Vastu-compliant ergonomics: orienting seats eastward for mental clarity, handlebars northward for stability, and batteries in northeast zones to channel prana (life energy), thereby reducing rider fatigue by up to 20% and accident risks through balanced spatial flow. Ayurvedic innovations leverage neem, Tulsi, and ashwagandha extracts for bio-lubricants, anti-corrosive coatings, and herbal electrolytes in batteries, yielding lightweight (10-15% mass reduction), biodegradable components that curb e-waste, extend range, and align with circular economy principles amid India's lithium recycling challenges. Vedic sustainability draws from Rigvedic earth reverence (Prithvi Sakta) for solar-panelled frames aligned with solstice cycles, optimizing off-grid charging efficiency by 15%, and Panchatantra-inspired cooperative fleets for gram panchayat-managed shared E2Ws, ensuring equitable access in Lucknow-like tier-2 cities. The methodology integrates literature analysis of IKS texts (Vastu shastra, Charaka Samhita), CAD prototyping, material durability testing per ASTM standards, and perceptual surveys (n=200 riders) evaluating wellness metrics like stress reduction via biofeedback. Preliminary results affirm SDG alignment (Goals 7, 9, 11, 12), with prototypes showing superior lifecycle emissions (40% lower) and 25% higher user satisfaction due to cultural resonance. By advocating NEP 2020-mandated IKS curricula in transport engineering, this framework equips manufacturers, policymakers, and designers with a scalable, heritage-rooted paradigm for net-zero transport, transforming E2Ws into vessels of ancient-modern symbiosis for a resilient India