Industry 5.0 requires close-proximity collaboration between humans and robots during CoKitting tasks, where physical fences impede operational flexibility. ISO/TS 15066:2016 Annex A.2 defines a dynamic Protective Separation Distance, S_p, yet practical software tools for visualizing the resulting Safety Index remain limited. This paper presents a Python Tkinter-based GUI that implements S_p and computes a real-time Safety Index SI = S_actual / S_p. For the representative case, the GUI calculates a Protective Separation Distance of 0.590 m. A built-in 15-second time-series simulation decreases the actual separation distance from 1.0 m to 0.3 m to demonstrate system response. Results from the GUI show SAFE operation for distances 1.0 m to 0.6 m where SI ≥ 1.0, a CAUTION state at 0.5 m where SI = 0.85 prompting speed reduction, and DANGER states below 0.4 m where SI < 0.7 triggering a protective stop. The developed GUI validates that Speed and Separation Monitoring enables fence-less collaboration by modulating robot behavior only when necessary. This tool provides an interactive means to test ISO 15066 logic before physical deployment, supporting human-centric manufacturing goals of Industry 5.0.

Keywords— Human-robot collaboration, ISO/TS 15066, Speed and Separation Monitoring, safety validation, GUI simulation, CoKitting, collaborative robotics, industrial safety.