Microorganisms that colonize internal plant tissues without endangering the host plant are known as endophytic microflora. These microbes are well-known for their advantageous qualities, including probiotic potential, stress tolerance, plant growth promotion, and antibacterial activity. Endophytic microorganisms from tomatoes (Solanum Lycopersicon), chilies (Capsicum annuum), Cucumis sativus, and capsicum (Capsicum annuum var. grossum) are isolated, characterized, and their probiotic potential is assessed. Nutrient agar and potato dextrose agar media were used for microbial isolation after healthy plant tissues, including roots, stems, leaves, and fruits, were gathered and surface sterilized. The morphological, microscopic, and biochemical traits of the isolated endophytes were used to identify them. Acid tolerance, bile salt tolerance, antibacterial activity, antibiotic susceptibility, and adhesion ability were among the tests used to assess the isolates' probiotic qualities. According to the findings, a number of isolates showed notable antibacterial activity against common pathogenic microorganisms as well as strong resistance to bile salts and acidic pH. These results imply that endophytic bacteria from frequently eaten vegetables could be viable probiotic candidates for use in the food, pharmaceutical, and agricultural industries. The study advances the investigation of sustainable and environmentally beneficial microbial resources.

Keyword: Endophytic microflora , probiotic , antibacterial activity , Nutrient agar , potato dextrose agar , Acid tolerance, bile salt tolerance.